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By DARIA LITVINOVA and DAVID RISING

MOSCOW (AP) —

The Kremlin on Tuesday rejected accusations of involvement in an alleged attack on Russian opposition leader Alexei Navalny who is in a coma in a German hospital, a day after

The politician’s allies say the Kremlin is behind the illness of its most prominent critic, with some demanding an investigation into whether Russian President Vladimir Putin was involved.

“These accusations absolutely cannot be true and are rather an empty noise,” Kremlin spokesman Dmitry Peskov told reporters on Tuesday. “We do not intend to take it seriously.”

Peskov saw no grounds for launching a criminal investigation into Navalny’s condition, saying that it could have been triggered by a variety of causes, and determining one should come first.

“If a substance (that caused the condition) is found, and if it is determined that it is poisoning, then there will be a reason for an investigation,” Peskov said.

Navalny, who is one of Putin’s fiercest critics, on Thursday and was taken to a hospital in the city of Omsk after the plane made an emergency landing.

Over the weekend he was transferred to the Charité hospital in Berlin, where doctors on Monday said they have found indications of “cholinesterase inhibitors” in his system.

Cholinesterase inhibitors act by blocking the breakdown of a key chemical in the body, acetycholine, that transmits signals between nerve cells. Navalny is being treated with the antidote atropine.

Navalny’s wife, Yulia Navalnaya, has been visiting her husband daily and made no comment to reporters as she arrived Tuesday.

Chancellor Angela Merkel personally offered Germany’s help in treating Navalny and, along with other European officials, has called for a full Russian investigation. U.S. Deputy Secretary of State Stephen Biegun’s was expected to bring the issue up during his visit to Russia starting Tuesday.

Charité said Monday that Navalny had undergone extensive examination by a team of physicians and that “clinical findings indicate poisoning with a substance from the group of cholinesterase inhibitors.”

Cholinesterase inhibitors are a broad range of substances that are found in several drugs, but also pesticides and nerve agents. Charité said the specific substance to which Navalny was exposed isn’t yet known but that a further series of comprehensive tests had been started.

Navalny’s spokeswoman, Kira Yarmysh, on Tuesday said the government’s reluctance to launch an investigation was expected.

“It was obvious that the crime would not be properly investigated and a culprit found. However, we all know perfectly well who that is,” Yarmysh tweeted.

Experts have cautioned that it is far to early to draw any conclusions about how the agent may have entered Navalny’s system, but note that Novichok, the Soviet-era nerve agent used to poison former Russian spy Sergei Skripal and his daughter in Britain, was a cholinesterase inhibitor.

“Cholinesterase inhibitor poisons can be given in many ways, they can be transported in many forms, and are very potent,” said Dr. Richard Parsons, a senior lecturer in biochemical toxicology at King’s College London. “This is why they are a favored method of poisoning people.”

Dr. Thomas Hartung, of the Johns Hopkins Bloomberg School of Public Health in Maryland, said such substances are easy to detect, even days and weeks after the poisoning, and that “we will know soon which substance was used.”

“The Novichok nerve agents, which was used in the 2018 poisoning of the Russian double agent Skripal in England, also belong to this category of substance,” he said. “I said at the time that the Russians could have have just left a business card at the crime scene, because the substances can be so clearly traced.”